Evaluation of Polycyclic Hydrocarbons Using Thermal Analysis and the Scientific and Biological Study of Isolated Bacteria Isolated from Contaminated Soil in Al-Zeit Street, (AL-Iraq Street), Benghazi, Libya

الملخص

In this study, soil samples taken from Al-Zeit Street (AL-Iraq), Benghazi, Libya, are examined for hydrocarbon pollution. Thermo Scientific's TSQ 8000 Evo Triple Quadrupole GC-MS/MS gas chromatography-mass spectrometry was used to analyse soil samples that were randomly selected from the surface, intermediate, and deep deposits. From C12 to C22, thirteen polycyclic aromatic hydrocarbons (PAHs) were found.include(Acenapthylene , Fluornen , Phenanthrene , Anthrathene , Pyrene , Benzo(a) anthrat , Chrysene , Benzo(b)Flou , Benzo(K)Flour Benzo(a)Pyrene , Indo(1,2,3 cd) P , Dibenzo(a,h)Anth , Benzo(g,h)Penyl). and using more sensitive methods like high-performance liquid chromatography or liquid spectral analysis, as well as the linear equation to determine the hydrocarbon contents. This is a connection between the soil, concentration, and analysis (e.g., chromatography's area under the curve). The findings show that hydrocarbon concentrations were below normal, most likely as a result of anaerobic and aerobic bacteria aiding in biodegradation. Concentrations above 50 ng/ml, however, indicate serious contamination and provide hazards to human health and the environment. The pH range of the soil was 8.00 to 9.96. encouraging the growth of microorganisms and the breakdown of hydrocarbons in the presence of nutritional limitations. The effectiveness of biodegradation was decreased by high salinity levels (73.3 to 1196 mg/L), which inhibited microbial enzymatic activity. The study found four bacterial strains that can break down hydrocarbons in anaerobic and aerobic environments: Bacillus, Clostridium, Staphylococcus, and Pseudomonas. These results demonstrate how bioremediation can be used to reduce petroleum contamination in oxygen-deficient conditions and in the presence of oxygen condition.