Prevalence and Isolation of Escherichia coli in Male and Female UTI Patients in Sabratha region

Authors

  • Salma .M. Elsweh Department of botany faculty of science – Zawia university Libya
  • Mohamed. O. Albasha قسم علم الحيوان، كلية العلوم، جامعة الزاوية، ليبيا
  • Alansary R. Elkhouly Department of biology faculty of science – Regdalin, Sabratha University, Libya.

Keywords:

UTI, E. coli, age groups

Abstract

Background: Urinary tract infections (UTIs) are among the most common bacterial infectious diseases encountered in clinical practice and account for significant morbidity and high medical costs. Escherichia coli is the most predominant pathogen causing 80-90% of community-acquired UTIs and 30-50% of nosocomial-acquired UTIs.

Objectives: the aim of this study is to evaluate the presence of  E.coli  in the Urinary tract infection (UTIs) in different age and sex patients in the area of the study.

Methods: The present study was conducted at Sabratha hospital during the period of October -December 2025. The samples were collected from UTIs patients (n= 629) (384) females and (245) males, who were registered in this research and the urine samples were taken aseptically for microbial culture. Patients were divided into 18 age groups.

Results: A total of patients (n= 629): (384) females and (245) males of urine were cultured on CLED agar, equal or more than 105 CFU/ml of a single potential pathogen was interpreted as positive. Out of these samples (236): (138) females and (98) males gave positive growth on CLED agar. On the other hand, total percentage of prevalence in female samples recorded (35.93%) while the highest percentage of infection from the total positive samples recorded in (0-5) age group (19.56%). In male samples the total percentage of prevalence recorded (40.0%) while the highest percentage of infection from the total positive samples recorded in (0-5) age group (37.75%).

Conclusion :This study showed a significant prevalence of urinary tract infections among the examined patients using CLED agar. Although more samples were collected from females, the proportion of positive cases was slightly higher in males. The highest infection rates were observed in children aged 0–5 years in both genders. These findings suggest increased susceptibility to infection in early childhood. Routine urine culture is essential for accurate diagnosis of UTIs. Early detection helps in effective treatment and prevention of complications.

Findings: The data revealed a predominance of the traditional production system, with 42.5% of respondents indicating that theoretical education is the prevailing model. Multiple regression analysis identified funding adequacy (β = 0.31, p < 0.001), academic staff efficiency (β = 0.27, p < 0.001), and infrastructure quality (β = 0.22, p = 0.002) as the three strongest predictors of perceived institutional productivity, collectively explaining 41.3% of the variance (R² = 0.413, F(5,432) = 57.42, p < 0.001). A strong consensus favored curriculum reform (75.3%, 95% CI: [71.3%, 79.4%]) and the adoption of a dual education model (75.3%) as primary development strategies.

Originality/Value: This paper presents an empirical classification of educational production systems in Libya, contributing to the limited body of research on higher education productivity in conflict-affected Arab states. We propose a measurable development model built on three pillars—institutional governance reform, effective industrial partnership development, and the establishment of a sustainable financing system—operationalized through Key Performance Indicators (KPIs) and phased implementation timelines

References

Akter T, Hossain MJ, Khan S, Sultana H, Fatema K, Sanjee S et al (2016). Isolation, identification and antimicrobial susceptibility pattern analysis of Escherichia coli isolated from clinical samples of Bangladesh. Asian Journal of Biomedical and Pharmaceutical Sciences, 2016, 6(54).

Apperloo-Renkema HZ, Van der Waaij BD and, Van der Waaij D.(1990). Determination of colonization resistance of the digestive tract by biotyping of Enterobacteriaceae. Epidemiol Infect. 1990;105(2):355–61.

Burhan O.(2017). Isolation and Identification of Urinary Tract

Infectious Bacteria and Exploring their Anti-drug Potential against Some Common Antibiotics. J Microb Biochem Technology. 2017; 9:6.

Croxen MA, Law RJ, Scholz R, Keeney KM, Wlodarska M, Finlay BB (2013). Recent advances in understanding enteric pathogenic Escherichia coli. Clinical microbiology reviews. 2013; 26(4):822-880.

Elkhouly, A. R., Ekram Almosy, & Nora Almosy. (2025). Study of bacterial and fungal infection of UTI patients in Sabratha and Jemil regions. Libyan Journal of Contemporary Academic Studies, 2(3), 50.: 3005-5970.

Faraji R, Sabzi F, and Center IAC (2012). Antimicrobial susceptibility of Escherichia coli isolated from patients with urinary tract infection referred to Imam Ali Hospital Kermanshah, Iran (2011). Life Sci J. 2012; 9(3):79-82.

Flores-Mireles, A.L.; Walker, J.N.; Caparon, M. and Hultgren, S.J(2015). Urinary tract infections: Epidemiology, mechanisms of infection and treatment options. Nat. Rev. Microbiol. 2015, 13, 269–284.

Foxman, B (2002). Epidemiology of urinary tract infections: incidence, morbidity, and economic costs. Am J Med 2002; 113:5S–13S

Foxman, B (2014). Urinary tract infection syndromes: Occurrence, recurrence, bacteriology, risk factors, and disease burden. Infect. Dis.

Clin. North. Am. 2014, 28, 1–13.

Hibbing ME, Fuqua C, Parsek MR and, Peterson SB (2010). Bacterial competition: surviving and thriving in the microbial jungle. Nat Rev Microbiol. 2010;8(1):15–25.

Rahman. S.R, Mushtaq Ahmad, Baitul Islam, Amir Ullah, Mujeeb ur Rahman, Zahid Khan, Haroon, Shahbaz Ahmad and Ikram Ullah. (2019). Isolation and identification of Escherichia coli from urine samples and their antibiotic susceptibility pattern. Journal of Entomology and Zoology Studies 2019; 7(3): 259-264.

Schappert SM (1999). Ambulatory care visits to physician offices, hospital

outpatient departments, and emergency departments: United States,

Vital Health Stat 13 1999; 143:i-iv, 1–39.

Stamm WE (2002). Scientific and clinical challenges in the management of

urinary tract infections. Am J Med 2002; 113:1S–4S.

Ullah A, Shah S, Almugadam B (2018). Prevalence of symptomatic urinary tract infections and antimicrobial susceptibility patterns of isolated uropathogens in kohat region of Pakistan. MOJ Biol Med. 2018; 3(4):85-9.

Weinstein MP, Towns ML, Quartey SM, et al (1990). The clinical significance of positive blood cultures in the 1990s: a prospective comprehensive evaluation of the microbiology, epidemiology, and outcome of bacteremia and fungemia in adults. Clin Infect Dis 1997; 24:584–602.

Mia Mosavie, Oliver Blandy, Elita Jauneikaite, Isabel Caldas, Matthew J. Ellington, Neil Woodford and Shiranee Sriskandan (2019). Sampling and diversity of Escherichia coli from the enteric microbiota in patients with Escherichia coli bacteraemia. BMC Res Notes (2019) 12:335

Alanazi1 M. Q, Fulwah Y. Alqahtani2 and Fadilah S. Aleanizy (2018). An evaluation of E. coli in urinary tract infection in emergency department at KAMC in Riyadh, Saudi Arabia: retrospective study. Ann Clin Microbiol Antimicrob (2018) 17:3.

Kahlmeter. G (2000). The ECO.SENS Project: a prospective, multinational, multicentre epidemiological survey of the prevalence and antimicrobial susceptibility of urinary tract pathogens—interim report. J Antimicrob Chemother. 2000;46(Suppl 1):15–22 (discussion 63–5).

Foxman. B (2010). The epidemiology of urinary tract infection. Nat Rev Urol. 2010;7(12):653–60.

Al Yousef. S.A, et al (2016). Clinical and laboratory profile of urinary tract infections associated with extended spectrum beta-lactamase producing Escherichia coli and Klebsiella pneumoniae. Ann Clin Lab Sci. 2016;46(4):393–400

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Published

2026-03-21

How to Cite

Salma .M. Elsweh, Mohamed. O. Albasha, & Alansary R. Elkhouly. (2026). Prevalence and Isolation of Escherichia coli in Male and Female UTI Patients in Sabratha region. Journal of Libyan Academy Bani Walid, 2(2), 172–181. Retrieved from https://journals.labjournal.ly/index.php/Jlabw/article/view/515

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Section

Applied Sciences