Characteristics of Semen Using Automated Analysis Techniques: A Comparative Study of Smokers and Non-Smokers

Hajer sliman ibrahim saidi; Gadeer alsharef bachir arhoma; Prof. fathi moftah abo saa

المؤلفون

Hajer sliman ibrahim saidi Department of Laboratory Sciences, Faculty of Medical Sciences and Technology, Tripoli, Libya.
Gadeer alsharef bachir arhoma Department of Laboratory Sciences, Faculty of Medical Sciences and Technology, Tripoli, Libya.
Prof. fathi moftah abo saa Dean, Faculty of Medical Sciences and Technology, Tripoli, Libya

الكلمات المفتاحية:

Semen، Male Fertility، Sperm Motility، Morphological Abnormalities، Reproductive Health

الملخص

This study aims to investigate the impact of cigarette smoking on semen characteristics using automated analysis techniques (Computer-Assisted Semen Analysis, CASA) by comparing semen parameters between smokers and non-smokers. A cross-sectional study design is proposed, involving 60 adult males (30 smokers, 30 non-smokers) aged 20-45 years, with exclusion criteria for chronic diseases or a history of infertility. Semen samples were collected according to World Health Organization (WHO) standards 1 and analyzed using a CASA system. Measured indicators included total sample volume, total sperm count and concentration, total and progressive motility, percentage of morphological abnormalities, liquefaction time, and pH level. Statistical analysis was performed using Student's T-test , with statistical significance set at P < 0.05. The results demonstrated statistically significant reductions in sperm concentration, total and progressive motility, and seminal volume in smokers compared to non-smokers. Conversely, smokers exhibited a statistically significant increase in the percentage of morphological abnormalities, alongside a higher pH and longer liquefaction time. These findings confirm the adverse effects of smoking on semen quality, reinforcing the importance of smoking cessation for male reproductive health.