Biological priming of potato cv. Spunta against Potato virus Y using Pseudomonas fluorescens and Trichoderma harzianum
Keywords:
potato, Potato virus Y, Pseudomonas fluorescens, Trichoderma harzianum, oxidative stress, induced resistance, total phenolic compoundsAbstract
Potato virus Y (PVY) is a major viral constraint to potato production. This study evaluated the response of potato cv. Spunta to PVY infection and assessed whether beneficial microbial treatments based on Pseudomonas fluorescens and Trichoderma harzianum could mitigate PVY-associated physiological injury under greenhouse conditions. Five treatments were compared: untreated control, PVY alone, preventive combined treatment with P. fluorescens + T. harzianum before PVY inoculation, preventive Pseudomonas treatment before PVY inoculation, and post-inoculation combined treatment. Biochemical traits were measured 3 and 12 days after the last treatment, and growth traits were recorded under greenhouse conditions. PVY-inoculated plants showed strong increases in catalase, peroxidase, polyphenol oxidase, and superoxide dismutase activities, together with higher H2O2 and MDA, and lower total protein, total phenolic compounds (TCP), biomass, length, and SPAD values. The preventive combined treatment produced the most favorable response, reducing H2O2 from 3200.50 to 980.40 mg l−1 at 3 days and from 1450.80 to 710.20 mg l−1 at 12 days, while lowering MDA from 45.60 to 20.10 mg l−1 and from 48.90 to 21.40 mg l−1, respectively. At 12 days, the same treatment increased total protein from 410.50 to
520.10 mg l−1 and TCP from 1.10 to 2.70 mg l−1 relative to PVY alone. Growth suppression was also markedly alleviated, with shoot fresh weight restored from 4.20 to 7.20 g and root fresh weight from 5.10 to 11.50 g. Overall, the dataset indicates that preventive microbial application, especially the preventive combined treatment, substantially mitigated PVY-associated oxidative, biochemical, and growth-related damage in potato cv. Spunta
References
Aebi, H. (1984). Catalase in vitro. In L. Packer (Ed.), Methods in enzymology (Vol. 105, pp. 121–126). Academic Press. https://doi.org/10.1016/S0076-6879(84)05016-3
Al-Ani, R. A., Athab, M. A., & Matny, O. N. (2013). Management of Potato virus Y (PVY) in potato by some biocontrol agents under field conditions. Journal of Pure and Applied Microbiology, 7(4), 2861–2865.
Bradford, M. M. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 72(1–2), 248–254. https://doi.org/10.1016/0003-2697(76)90527-3
Chance, B., & Maehly, A. C. (1955). Assay of catalases and peroxidases. Methods in Enzymology, 2, 764–775. https://doi.org/10.1016/S0076-6879(55)02300-8
De Vleesschauwer, D., Djavaheri, M., Bakker, P. A. H. M., & Höfte, M. (2008). Pseudomonas fluorescens WCS374r-induced systemic resistance in rice against Magnaporthe oryzae is based on pseudobactin-mediated priming for a salicylic acid-repressible multifaceted defense response. Plant Physiology, 148(4), 1996–2012.
https://doi.org/10.1104/pp.108.127878
Deja-Sikora, E., Werner, A., & Hrynkiewicz, K. (2023). AMF species do matter: Rhizophagus irregularis and Funneliformis mosseae affect healthy and PVY-infected Solanum tuberosum L. in a different way. Frontiers in Microbiology, 14, 1127278.
https://doi.org/10.3389/fmicb.2023.1127278
Gallou, A., Cranenbrouck, S., & Declerck, S. (2009). Trichoderma harzianum elicits defence response genes in roots of potato plantlets challenged by Rhizoctonia solani. European Journal of Plant Pathology, 124(2), 219–230.
https://doi.org/10.1007/s10658-008-9407-x
García-Marcos, A., Pacheco, R., Martíáñez, J., González-Jara, P., Díaz-Ruíz, J. R., & Tenllado, F. (2009). Transcriptional changes and oxidative stress associated with the synergistic interaction between Potato virus X and Potato virus Y and their relationship with symptom expression. Molecular Plant-Microbe Interactions, 22(11), 1431–1444.
https://doi.org/10.1094/MPMI-22-11-1431
Giannopolitis, C. N., & Ries, S. K. (1977). Superoxide dismutases: I. Occurrence in higher plants. Plant Physiology, 59(2), 309–314.
https://doi.org/10.1104/pp.59.2.309
Heath, R. L., & Packer, L. (1968). Photoperoxidation in isolated chloroplasts: I. Kinetics and stoichiometry of fatty acid peroxidation. Archives of Biochemistry and Biophysics, 125(1), 189–198. https://doi.org/10.1016/0003-9861(68)90654-1
Hernández, J. A., Gullner, G., Clemente-Moreno, M. J., Künstler, A., Juhász, C., Diaz-Vivancos, P., & Király, L. (2016). Oxidative stress and antioxidative responses in plant-virus interactions. Physiological and Molecular Plant Pathology, 94, 134–148.
https://doi.org/10.1016/j.pmpp.2015.09.001
Hull, R. (2002). Matthews' plant virology (4th ed.). Academic Press. https://doi.org/10.1016/B978-0-12-361160-4.X5050-6
Mayer, A. M., & Harel, E. (1991). Phenoloxidases and their significance in fruit and vegetables. In P. F. Fox (Ed.), Food enzymology (pp. 373–398). Elsevier.
Patterson, B. D., MacRae, E. A., & Ferguson, I. B. (1984). Estimation of hydrogen peroxide in plant extracts using titanium(IV). Analytical Biochemistry, 139(2), 487–492. https://doi.org/10.1016/0003-2697(84)90039-3
Pieterse, C. M. J., Zamioudis, C., Berendsen, R. L., Weller, D. M., Van Wees, S. C. M., & Bakker, P. A. H. M. (2014). Induced systemic resistance by beneficial microbes. Annual Review of Phytopathology, 52, 347–375.
https://doi.org/10.1146/annurev-phyto-082712-102340
Singleton, V. L., & Rossi, J. A. (1965). Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. American Journal of Enology and Viticulture, 16(3), 144–158.
Vitti, A., Pellegrini, E., Nali, C., Lovelli, S., Sofo, A., Valerio, M., & Nuzzaci, M. (2016). Trichoderma harzianum T-22 induces systemic resistance in tomato infected by Cucumber mosaic virus. Frontiers in Plant Science, 7, 1520.
https://doi.org/10.3389/fpls.2016.01520
Yu, Y., Gui, Y., Li, Z., Jiang, C., Guo, J., & Niu, D. (2022). Induced systemic resistance for improving plant immunity by beneficial microbes. Plants, 11(3), 386.
